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KMID : 0545120060160091429
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Journal of Microbiology and Biotechnology
2006 Volume.16 No. 9 p.1429 ~ p.1433
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Expression and Purification of a Cathelicidin-Derived Antimicrobial Peptide, CRAMP
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Park Eu-Jin
Chae Young-Kee
Lee Byoung-Jae
Kim Yang-Mee
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Abstract
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Application of recombinant protein production and particularly their isotopic enrichment has stimulated development of a range of novel multidimensional heteronuclear NMR techniques. Peptides in most cases are amenable to assignment and structure determination without the need for isotopic labeling. However, there are many cases where the availability of and/or labeled peptides is useful to study the structure of peptides with more than 30 residues and the interaction between peptides and membrane. CRAMP (Cathelicidin-Related AntiMicrobial Peptide) was identified from a cDNA clone derived from mouse femoral marrow cells as a member of cathelicidin-derived antimicrobial peptides. CRAMP was successfully expressed as a GST-fused form in E. coli and purified using affinity chromatography and reverse-phase chromatography. The yield of the CRAMP was 1.5 mg/l 1. According to CD spectra, CRAMP adopted -helical conformation in membrane-mimetic environments. Isotope labeling of CRAMP is expected to make it possible to study the structure and dynamic properties of CRAMP in various membrane systems.
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KEYWORD
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antimicrobial peptides, purification, NMR, CRAMP, expression
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